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Essays on Infinite Lifespans
Brian Wowk
New breakthroughs in reducing the toxicity of vitrifica-
tion solutions [20], and in adding synthetic ice blocking
molecules [21;22] continue to push the field forward.
In 2004, successful transplantation of rabbit kidneys after
cooling to a temperature of -45°C (-49°F) was reported.
[23] These kidneys were prevented from freezing by replac-
ing more than half of the water inside them with vitrification
chemicals. Amazingly, organs can survive this extreme treat-
ment if the chemicals are introduced and removed quickly at
low temperature.
Reversible vitrification of major organs is a reasonable pros-
pect within this decade. What about vitrification of whole
animals? This is a much more difficult problem. Some organs,
such as the kidney and brain, are privileged organs for vit-
rification because of their high blood flow rate. This allows
vitrification chemicals to enter and leave them quickly before
there are toxic effects. Most other tissues would not survive
the long chemical exposure time required to absorb a suffi-
cient concentration to prevent freezing.
It is useful to distinguish between reversible vitrification and
morphological vitrification. Reversible vitrification is vitrifi-
cation in which tissue recovers from the vitrification process
in a viable state. Morphological vitrification is vitrification in
which tissue is preserved without freezing, with good structural
preservation, but in which key enzymes or other biomolecules
are damaged by the vitrification chemicals. Morphological
vitrification of a kidney was photographically demonstrated
in Fahys original vitrification paper [17], but 20 years later
reversible kidney vitrification is still being pursued.
Given this background, what are the prospects of reversibly
vitrifying a whole human being? It is theoretically possible,
but the prospects are still distant. Morphological vitrification
of most organs and tissues in the body may now be possible,
but moving from morphological vitrification to reversible